Ribonuclease T (RNase T, exonuclease T, exo T ) is a ribonuclease enzyme that acts upon the maturation of transport and ribosomal RNAs in bacteria [2] , as well as in DNA repair processes [3] .
| Ribonuclease T | |
|---|---|
 Dimeric ribonuclease T (individual enzyme monomers are shown in green and blue) in combination with DNA (shown in orange), PDB ID 3NH1. [one] | |
| Identifiers | |
| Symbol | rnt |
| Pfam | PF00929 |
| Interpro | IPR013520 |
| SMART | SM00479 |
| Available protein structures | |
| Pfam | the structure |
| PDB | RCSB PDB ; PDBe ; PDBj |
| PDBsum | 3D model |
Ribonuclease T is part of the DnaQ exonuclease family . It acts on the 3'-end of single-stranded nucleic acids and can cleave both DNA and RNA with high specificity discriminating the cytosine residue at the 3'-end of the substrate [1] [2] .
See also
- Ribonuclease
Notes
- ↑ 1 2 Hsiao YY, Duh Y., Chen YP, Wang YT, Yuan HS How an exonuclease decides where to stop in trimming of nucleic acids: crystal structures of RNase T-product complexes (Eng.) // Nucleic Acids Research : journal. - 2012 .-- September ( vol. 40 , no. 16 ). - P. 8144-8154 . - DOI : 10.1093 / nar / gks548 . - PMID 22718982 .
- ↑ 1 2 Zuo Y., Deutscher MP The physiological role of RNase T can be explained by its unusual substrate specificity (English) // The Journal of Biological Chemistry : journal. - 2002 .-- August ( vol. 277 , no. 33 ). - P. 29654-29661 . - DOI : 10.1074 / jbc.M204252200 . - PMID 12050169 .
- ↑ Hsiao YY, Fang WH, Lee CC, Chen YP, Yuan HS Structural insights into DNA repair by RNase T - an exonuclease processing 3 'end of structured DNA in repair pathways (English) // PLoS Biology : journal. - 2014 .-- March ( vol. 12 , no. 3 ). - P. e1001803 . - DOI : 10.1371 / journal.pbio.1001803 . - PMID 24594808 .