Green janus (2-diethylamino-3,6-dimethyl-9-phenylphenazonium-7-azo-4′-dimethylaniline chloride) is an organic compound , a dye belonging to diazotized safranins and having the chemical formula C 32 H 35 N 6 Cl. A dark green powder giving a blue aqueous solution. It is used in microscopy for intravital staining , in analytical chemistry - as a reagent for tin and as a redox indicator .
| Janus green | |
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| General | |
| Chem. formula | C₃₀H₃₁ClN₆ |
| Physical properties | |
| Molar mass | 539.12 g / mol |
| Classification | |
| Reg. CAS number | |
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| Reg. EINECS number | |
| Smiles | |
| Inchi | |
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| Chebi | |
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Synonyms: Janus green B, diethylsafranin-7-azo-4′-dimethylaniline chloride, union green B, CI 11050 [1] .
Content
- 1 Properties
- 1.1 Analytical definition
- 2 Application
- 3 notes
- 4 Literature
Properties
Crystals with a color ranging from dark green to brown. Molar mass of 539.12 g / mol. It is soluble in water, at the same time it forms a solution of blue color, it is poorly soluble in ethanol [1] .
Analytical Definition
It gives olive green staining in concentrated sulfuric acid [1] .
An aqueous solution with the addition of hydrochloric acid gives a blue precipitate, with the addition of alkalis - black [1] .
Application
In microscopy, it is mainly used for intravital staining of mitochondria, it is also used for staining blood and white blood cells in particular, protozoa and fungi. Finds application in fluorescence microscopy [1] .
In analytical chemistry, it is used to determine tin as an oxidation-reduction indicator with a transition from blue to colorless with a potential of E 1/2 = −0.23 V at pH 7.0 [1] .
Notes
- ↑ 1 2 3 4 5 6 Freistatt, 1980 , p. 462-463.
Literature
- Freistat D.S. Reagents and preparations for microscopy. - M .: Chemistry, 1980 .-- 480 p.