Kinetochore protein Nuf2 ( Eng. Kinetochore protein Nuf2 ) is a protein of the external kinetochore plate involved in cell division. The human gene product NUF2 [1] [2] [3] .
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| , CDCA1, CT106, NUF2R, NDC80 kinetochore complex component, NDC80 kinetochore complex component NUF2 component of NDC80 kinetochore complex | |||||||||||||
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Functions
The Nuf2 protein , together with SPC25 and SPC24, is included in the NDC80 heterotetrameric complex of the outer kinetochore plate associated with the centromere. It plays a critical role in the separation of chromosomes and spindle activity during cell division [3] .
Structure
The protein consists of 464 amino acids, a molecular weight of 54.3 kDa. The N-terminal portion (1–385) interacts with the N-terminal portion of NDC80 , the C-terminal portion (386–464) is connected to the C-terminus of NDC8 0 and to the SPC25 / SPC24 subcomplex .
Notes
- ↑ Wigge PA, Kilmartin JV The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation (Eng.) // J Cell Biol : journal. - 2001 .-- March ( vol. 152 , no. 2 ). - P. 349-360 . - DOI : 10.1083 / jcb.152.2.349 . - PMID 11266451 .
- ↑ Nabetani A., Koujin T., Tsutsumi C., Haraguchi T., Hiraoka Y. A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint ( English) // Chromosoma: journal. - 2001 .-- October ( vol. 110 , no. 5 ). - P. 322—334 . - DOI : 10.1007 / s004120100153 . - PMID 11685532 .
- ↑ 1 2 Entrez Gene: NUF2 NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) .
Literature
- DeLuca JG, Moree B., Hickey JM, etal. hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. (English) // J. Cell Biol. : journal. - 2003. - Vol. 159 , no. 4 . - P. 549-555 . - DOI : 10.1083 / jcb.200208159 . - PMID 12438418 .
- Strausberg RL, Feingold EA, Grouse LH, etal. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (Eng.) // Proceedings of the National Academy of Sciences of the United States of America : journal. - 2003. - Vol. 99 , no. 26 . - P. 16899-16903 . - DOI : 10.1073 / pnas.242603899 . - PMID 12477932 .
- Tien AC, Lin MH, Su LJ, etal. Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model. (English) // Mol. Cell. Proteomics : journal. - 2004. - Vol. 3 , no. 1 . - P. 93-104 . - DOI : 10.1074 / mcp.M300072-MCP200 . - PMID 14602875 .
- DeLuca JG, Howell BJ, Canman JC, etal. Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. (English) // Curr. Biol. : journal. - 2004. - Vol. 13 , no. 23 . - P. 2103-2109 . - DOI : 10.1016 / j.cub.2003.10.056 . - PMID 14654001 .
- Bharadwaj R., Qi W., Yu H. Identification of two novel components of the human NDC80 kinetochore complex. (English) // J. Biol. Chem. : journal. - 2004. - Vol. 279 , no. 13 . - P. 13076-13085 . - DOI : 10.1074 / jbc.M310224200 . - PMID 14699129 .
- Ota T., Suzuki Y., Nishikawa T., etal. Complete sequencing and characterization of 21,243 full-length human cDNAs. (Eng.) // Nat. Genet. : journal. - 2004. - Vol. 36 , no. 1 . - P. 40-5 . - DOI : 10.1038 / ng1285 . - PMID 14702039 .
- Joseph J., Liu ST, Jablonski SA, etal. The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo. (English) // Curr. Biol. : journal. - 2004. - Vol. 14 , no. 7 . - P. 611-617 . - DOI : 10.1016 / j.cub.2004.03.03.031 . - PMID 15062103 .
- Stucke VM, Baumann C., Nigg EA Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1. (English) // Chromosoma: journal. - 2005. - Vol. 113 , no. 1 . - P. 1-15 . - DOI : 10.1007 / s00412-004-0288-2 . - PMID 15235793 .
- Meraldi P., Draviam VM, Sorger PK Timing and checkpoints in the regulation of mitotic progression. (English) // Dev. Cell : journal. - 2004. - Vol. 7 , no. 1 . - P. 45-60 . - DOI : 10.1016 / j.devcel.2004.06.06.006 . - PMID 15239953 .
- Cheeseman IM, Niessen S., Anderson S., etal. A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension. (Eng.) // Genes Dev. : journal. - 2004. - Vol. 18 , no. 18 . - P. 2255-2268 . - DOI : 10.1101 / gad.1234104 . - PMID 15371340 .
- Gerhard DS, Wagner L., Feingold EA, etal. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (Eng.) // Genome Res. : journal. - 2004. - Vol. 14 , no. 10B . - P. 2121-2127 . - DOI : 10.1101 / gr.2596504 . - PMID 15489334 .
- DeLuca JG, Dong Y., Hergert P., etal. Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites. (Eng.) // Molecular Biology of the Cell : journal. - 2005. - Vol. 16 , no. 2 . - P. 519-531 . - DOI : 10.1091 / mbc.E04-09-0852 . - PMID 15548592 .
- Ciferri C., De Luca J., Monzani S., etal. Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. (English) // J. Biol. Chem. : journal. - 2005. - Vol. 280 , no. 32 . - P. 29088-29095 . - DOI : 10.1074 / jbc.M504070200 . - PMID 15961401 .
- Nousiainen M., Silljé HH, Sauer G., etal. Phosphoproteome analysis of the human mitotic spindle. (Eng.) // Proceedings of the National Academy of Sciences of the United States of America : journal. - 2006. - Vol. 103 , no. 14 . - P. 5391-5396 . - DOI : 10.1073 / pnas.0507066103 . - PMID 16565220 .
- Gregory SG, Barlow KF, McLay KE, etal. The DNA sequence and biological annotation of human chromosome 1. (Eng.) // Nature: journal. - 2006. - Vol. 441 , no. 7091 . - P. 315-321 . - DOI : 10.1038 / nature04727 . - PMID 16710414 .
- Olsen JV, Blagoev B., Gnad F., etal. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (English) // Cell : journal. - Cell Press , 2006. - Vol. 127 , no. 3 . - P. 635-648 . - DOI : 10.1016 / j.cell.2006.09.09.026 . - PMID 17081983 .
- Ewing RM, Chu P., Elisma F., etal. Large-scale mapping of human protein-protein interactions by mass spectrometry. (English) // Mol. Syst. Biol. : journal. - 2007. - Vol. 3 , no. 1 . - P. 89 . - DOI : 10.1038 / msb4100134 . - PMID 17353931 .
- Liu D., Ding X., Du J., etal. Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. (English) // J. Biol. Chem. : journal. - 2007. - Vol. 282 , no. 29 . - P. 21415-21424 . - DOI : 10.1074 / jbc.M609026200 . - PMID 17535814 .