HepG2 is a human hepatocellular carcinoma cell line derived from the liver tissue of a 15-year-old white American man with well-differentiated hepatocellular carcinoma. In morphological studies, the line shows a pronounced epithelial phenotype, the modal number of chromosomes is 55 and are not tumorigenic for nude mice [1] . Line cells secrete many major plasma proteins, such as albumin , transferrin , fibrinogen , alpha-2-macroglobulin, alpha-1-antitrypsin, and plasminogen . The line successfully proliferated in large-scale cultivation systems. No surface antigens of hepatitis B virus were detected in the cells of the line.
Currently, the cell line is used in proteomic research [2] and material testing to create tissue-engineering structures [3] .
See also
- Huh7
Notes
- ↑ "Hep G2", American Type Culture Collection .
- ↑ EV Poverennaya, OI Kiseleva, EA Ponomarenko, SN Naryzhny, VG Zgoda. Multiomics study of HepG2 cell line proteome (Eng.) // Biomeditsinskaya Khimiya. - 2017 .-- T. 63 , no. 5 . - S. 373–378 . - ISSN 2310-6905 2310-6972, 2310-6905 . - DOI : 10.18097 / pbmc20176305373 .
- ↑ Onishchenko N.A., Gulay Yu.S., Shagidulin M.Yu., Nikolskaya A.O., Bashkina L.V. DEVELOPMENT OF IMPLANTED CELL AND TISSUE ENGINEERING CONSTRUCTIONS OF AUXILIARY LIVER FOR TREATMENT OF HEPATIC INSUFFICIENCY // Genes and Cells. - 2015. - No. 1 . - S. 6-17 .