The Trim-Away method is a method of rapid forced highly selective (selective) degradation of endogenous proteins in mammalian cells , which does not require preliminary modification of the genome or matrix RNA . Since the selectivity of the Trim-Away method is based on the use of antibodies , it can be applied to a wide range of target proteins using ready-made monoclonal antibodies . Trim-Away allows you to study the function of the protein in a variety of cell types [1] [2] . Designed by Melina Schuh in collaboration with Dean Clift [3] .
Principle of Operation
The method is based on the use of a ubiquitin ligase , TRIM21 , which binds with high affinity to the domain of a crystallizing fragment of immunoglobulin Fc antibodies [4] . During infection, TRIM21 transports the ubiquitin proteasome system to antibody-associated pathogens, leading to their destruction.
Notes
- ↑ Clift D. , McEwan WA , Labzin LI , Konieczny V. , Mogessie B. , James LC , Schuh M. A Method for the Acute and Rapid Degradation of Endogenous Proteins. (English) // Cell. - 2017. - DOI : 10.1016 / j.cell.2017.10.10.033 . - PMID 29153837 .
- ↑ How to destroy any protein in any cell . ScienceDaily, 16 November 2017, Max-Planck-Gesellschaft.
- ↑ https://www2.mrc-lmb.cam.ac.uk/melina-schuh-awarded-the-embo-gold-medal/
- ↑ James LC , Keeble AH , Khan Z. , Rhodes DA , Trowsdale J. Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function. (Eng.) // Proceedings of the National Academy of Sciences of the United States of America. - 2007. - Vol. 104, no. 15 . - P. 6200-6205. - DOI : 10.1073 / pnas.0609174104 . - PMID 17400754 .