The checkpoint protein HUS1 is a protein encoded in humans by the HUS1 gene [1] [2] .
| HUS1 checkpoint protein homologue ( Schiz. Pombe ) | |||||||||||||
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 Presented based on PDB . | |||||||||||||
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| Symbol | ; hHUS1 | ||||||||||||
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Functions
The protein encoded by this gene is a component of the evolutionarily conserved, genotoxin-activated protein complex of the control point, which is involved in stopping the cell cycle in response to DNA damage. This protein forms a complex with heterotrimeric proteins of the checkpoint RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of the RAD17 protein and four small subunits (RFC), which fixes the combined complex to chromatin . Chromatin binding induced by DNA damage depends on the activity of the ATM kinase , and is considered an early event in the signaling pathway triggered at a reference point [3] .
Interactions with other proteins
HUS1 has been found to interact with RAD9A [4] [5] [6] [7] , RAD17 [8] [9] , HDAC1 [10] , [5] [11] and [12] .
Notes
- ↑ Dean FB, Lian L., O'Donnell M. cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster (Eng.) // Genomics : journal. - Academic Press , 1999 .-- February ( vol. 54 , no. 3 ). - P. 424-436 . - DOI : 10.1006 / geno.1998.5587 . - PMID 9878245 .
- ↑ Kostrub CF, Knudsen K., Subramani S., Enoch T. Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage (Eng.) // EMBO J. : journal. - 1998 .-- June ( vol. 17 , no. 7 ). - P. 2055-2066 . - DOI : 10.1093 / emboj / 17.7.2055 . - PMID 9524127 .
- ↑ Entrez Gene: HUS1 HUS1 checkpoint homolog (S. pombe) .
- ↑ Dufault, Vanessa M; Oestreich Andrea J; Vroman Benjamin T; Karnitz Larry M. Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene (Eng.) // Genomics : journal. - United States: Academic Press , 2003. - December ( vol. 82 , no. 6 ). - P. 644-651 . - ISSN 0888-7543 . - DOI : 10.1016 / S0888-7543 (03) 00200-3 . - PMID 14611806 .
- ↑ 1 2 Volkmer, E; Karnitz L M. Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex (Eng.) // J. Biol. Chem. : journal. - UNITED STATES, 1999 .-- January ( vol. 274 , no. 2 ). - P. 567-570 . - ISSN 0021-9258 . - DOI : 10.1074 / jbc.274.2.567 . - PMID 9872989 .
- ↑ Griffith, Jack D; Lindsey-Boltz Laura A., Sancar Aziz. Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray / low voltage microscopy (Eng.) // J. Biol. Chem. : journal. - United States, 2002. - May ( vol. 277 , no. 18 ). - P. 15233-15236 . - ISSN 0021-9258 . - DOI : 10.1074 / jbc.C200129200 . - PMID 11907025 .
- ↑ Hirai, Itaru; Wang Hong-Gang. A role of the C-terminal region of human Rad9 (hRad9) in nuclear transport of the hRad9 checkpoint complex (Eng.) // J. Biol. Chem. : journal. - United States, 2002. - July ( vol. 277 , no. 28 ). - P. 25722-25727 . - ISSN 0021-9258 . - DOI : 10.1074 / jbc.M203079200 . - PMID 11994305 .
- ↑ Bermudez, Vladimir P; Lindsey-Boltz Laura A., Cesare Anthony J., Maniwa Yoshimasa, Griffith Jack D., Hurwitz Jerard, Sancar Aziz. Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro (English) // Proceedings of the National Academy of Sciences of the United States of America : journal. - United States, 2003. - February ( vol. 100 , no. 4 ). - P. 1633-1638 . - ISSN 0027-8424 . - DOI : 10.1073 / pnas.0437927100 . - PMID 12578958 .
- ↑ Rauen, M; Burtelow MA; Dufault VM Karnitz L M. The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9 (Eng.) // J. Biol. Chem. : journal. - UNITED STATES, 2000 .-- September ( vol. 275 , no. 38 ). - P. 29767-29771 . - ISSN 0021-9258 . - DOI : 10.1074 / jbc.M005782200 . - PMID 10884395 .
- ↑ Cai, RL; Yan-Neale Y; Cueto MA; Xu H; Cohen D. HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2 / M checkpoint Rad proteins (Eng.) // J. Biol. Chem. : journal. - UNITED STATES, 2000 .-- September ( vol. 275 , no. 36 ). - P. 27909—27916 . - ISSN 0021-9258 . - DOI : 10.1074 / jbc.M000168200 . - PMID 10846170 .
- ↑ Hang, Haiying; Zhang Yuzhu; Dunbrack Roland L; Wang Cuidong; Lieberman Howard B. Identification and characterization of a paralog of human cell cycle checkpoint gene HUS1 (Eng.) // Genomics : journal. - United States: Academic Press , 2002. - April ( vol. 79 , no. 4 ). - P. 487-492 . - ISSN 0888-7543 . - DOI : 10.1006 / geno.2002.6737 . - PMID 11944979 .
- ↑ Komatsu, K; Wharton w; Hang H; Wu C; Singh S; Lieberman HB; Pledger WJ; Wang H G. PCNA interacts with hHus1 / hRad9 in response to DNA damage and replication inhibition (Eng.) // Oncogene : journal. - ENGLAND, 2000. - November ( vol. 19 , no. 46 ). - P. 5291-5297 . - ISSN 0950-9232 . - DOI : 10.1038 / sj.onc.1203901 . - PMID 11077446 .
Literature
- Dominguez O; Ashhab Y; Sabater L; Belloso, Eva; Caro, Pepi; Pujol-Borrell, Ricardo. Cloning of ARE-containing genes by AU-motif-directed display (Eng.) // Genomics : journal. - Academic Press , 1999. - Vol. 54 , no. 2 - P. 278-286 . - DOI : 10.1006 / geno.1998.5548 . - PMID 9828130 .
- Volkmer E., Karnitz LM Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex (Eng.) // J. Biol. Chem. : journal. - 1999. - Vol. 274 , no. 2 - P. 567-570 . - DOI : 10.1074 / jbc.274.2.567 . - PMID 9872989 .
- St Onge RP, Udell CM, Casselman R., Davey S. The Human G2 Checkpoint Control Protein hRAD9 Is a Nuclear Phosphoprotein That Forms Complexes with hRAD1 and hHUS1 (Eng.) // Molecular Biology of the Cell : journal. - 1999. - Vol. 10 , no. 6 - P. 1985-1995 . - DOI : 10.1091 / mbc.10.6.1985 . - PMID 10359610 .
- Hang H., Lieberman HB Physical interactions among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression (Eng.) // Genomics : journal. - Academic Press , 2000. - Vol. 65 , no. 1 . - P. 24-33 . - DOI : 10.1006 / geno.2000.6142 . - PMID 10777662 .
- Cai RL; Yan-Neale Y; Cueto MA; Xu, H; Cohen, D. HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2 / M checkpoint Rad proteins (Eng.) // J. Biol. Chem. : journal. - 2000. - Vol. 275 , no. 36 - P. 27909—27916 . - DOI : 10.1074 / jbc.M000168200 . - PMID 10846170 .
- Burtelow MA, Kaufmann SH, Karnitz LM Retention of the human Rad9 checkpoint complex in extraction-resistant nuclear complexes after DNA damage (Eng.) // J. Biol. Chem. : journal. - 2000. - Vol. 275 , no. 34 - P. 26343-26348 . - DOI : 10.1074 / jbc.M001244200 . - PMID 10852904 .
- Rauen M., Burtelow MA, Dufault VM, Karnitz LM The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9 (Eng.) // J. Biol. Chem. : journal. - 2000. - Vol. 275 , no. 38 - P. 29767-29771 . - DOI : 10.1074 / jbc.M005782200 . - PMID 10884395 .
- Komatsu K; Wharton w; Hang H; Wu, Chun; Singh, Sujay; Lieberman, Howard B; Pledger, WJ; Wang, Hong-Gang. PCNA interacts with hHus1 / hRad9 in response to DNA damage and replication inhibition (Eng.) // Oncogene : journal. - 2000. - Vol. 19 , no. 46 . - P. 5291-5297 . - DOI : 10.1038 / sj.onc.1203901 . - PMID 11077446 .
- Xiang SL; Kumano T; Iwasaki SI; Sun, Xiangao; Yoshioka, Kastuji; Yamamoto, Ken-chi. The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9 (Eng.) // Biochem. Biophys. Res. Commun. : journal. - 2001. - Vol. 287 , no. 4 - P. 932-940 . - DOI : 10.1006 / bbrc.2001.5685 . - PMID 11573955 .
- Griffith JD, Lindsey-Boltz LA, Sancar A. Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray / low voltage microscopy ( J. ) // J. Biol. Chem. : journal. - 2002. - Vol. 277 , no. 18 . - P. 15233-15236 . - DOI : 10.1074 / jbc.C200129200 . - PMID 11907025 .
- Kinzel B; Hall J; Natt f; Weiler, Jan; Cohen, Dalia. Downregulation of Hus1 by antisense oligonucleotides enhances the sensitivity of human lung carcinoma cells to cisplatin (Eng.) // Cancer : journal. - 2002. - Vol. 94 , no. 6 - P. 1808-1814 . - DOI : 10.1002 / cncr.10383 . - PMID 11920544 .
- Hang H; Zhang Y; Dunbrack RL; Wang, Cuidong; Lieberman, Howard B. Identification and characterization of a paralog of human cell cycle checkpoint gene HUS1 // Genomics : journal. - Academic Press , 2002. - Vol. 79 , no. 4 - P. 487-492 . - DOI : 10.1006 / geno.2002.6737 . - PMID 11944979 .
- Roos-Mattjus P; Vroman BT; Burtelow MA; Rauen, M; Eapen, AK; Karnitz, LM Genotoxin-induced Rad9-Hus1-Rad1 (9-1-1) chromatin association is an early checkpoint signaling event (Eng.) // J. Biol. Chem. : journal. - 2003. - Vol. 277 , no. 46 . - P. 43809-43812 . - DOI : 10.1074 / jbc.M207272200 . - PMID 12228248 .
- Strausberg RL; Feingold EA; Grouse lh; Derge, JG; Klausner, RD; Collins, FS; Wagner, L; Shenmen, CM; Schuler, GD Generation and the cDNA sequences (English) // Proceedings of the United States of America : journal. - 2003. - Vol. 99 , no. 26 - P. 16899-16903 . - DOI : 10.1073 / pnas.242603899 . - PMID 12477932 .
- Bermudez VP; Lindsey-Boltz LA; Cesare AJ; Maniwa, Y; Griffith, JD; Hurwitz, J; Sancar, A. Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro (English) // Proceedings of the National Academy of Sciences of the United States of America : journal. - 2003. - Vol. 100 , no. 4 - P. 1633-1638 . - DOI : 10.1073 / pnas.0437927100 . - PMID 12578958 .
- Scherer SW; Cheung J; MacDonald JR; Osborne, LR; Nakabayashi, K; Herbrick, JA; Carson, AR; Parker-Katiraee, L; Skaug, J. Human Chromosome 7: DNA Sequence and Biology (Eng.) // Science. - 2003. - Vol. 300 , no. 5620 . - P. 767-772 . - DOI : 10.1126 / science.1083423 . - PMID 12690205 .
- Hopkins KM; Wang X; Berlin A; Hang, H; Thaker, HM; Lieberman, HB Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue (Eng.) // Cancer Res. : journal. - 2003. - Vol. 63 , no. 17 . - P. 5291-5298 . - PMID 14500360 .
- Dufault VM, Oestreich AJ, Vroman BT, Karnitz LM Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene (Eng.) // Genomics : journal. - Academic Press , 2004. - Vol. 82 , no. 6 - P. 644-651 . - DOI : 10.1016 / S0888-7543 (03) 00200-3 . - PMID 14611806 .