The mannose receptor , type C lectin , is located on the surface of macrophages and dendritic cells , as well as some skin cells, such as skin fibroblasts and keratinocytes . [1] [2] Included in the family of endocytic receptors along with Endo180 (CD280) , M-type PLA2R and DEC-205 (CD205) . [3]
| Mannose receptor, type C 1 | |
|---|---|
| Designations | |
| Characters | MRC1 ; bA541I19.1, CD206, CLEC13D, CLEC13DL |
| Entrez gene | 4360 |
| Hgnc | 7228 |
| Omim | 153618 |
| Refseq | NM_002438 |
| Uniprot | P22897 |
| Other data | |
| Locus | 10th hr , 10p13 |
| Mannose receptor, C type 2 | |
|---|---|
| Designations | |
| Characters | MRC2 ; CD280, CLEC13E, ENDO180, KIAA0709 |
| Entrez gene | 9902 |
| Hgnc | 16875 |
| Omim | 612264 |
| Refseq | NM_006039 |
| Uniprot | Q9UBG0 |
| Other data | |
| Locus | 17th ridge , 17q23 |
The mannose receptor recognizes terminal residues of mannose , N-acetylglucosamine and fucose on glycan chains of proteins [4] located on the surface of certain microorganisms, and plays a role in innate and acquired immunity. In addition, the mannose receptor is involved in the removal of glycoproteins from the bloodstream, including sulfated glycopeptide hormones and glycoproteins released as a result of pathological processes. [5] The mannose receptor is constantly recycled between the plasma membrane and endosomes in a clathrin- dependent manner. [6] There are 2 types of mannose receptor, the products of the genes MRC1 (CD206) and MRC2 (CD280).
Content
Structure
The mannose receptor refers to type 1 transmembrane proteins with an extracellular N-terminus and an intracellular C-terminus. It is synthesized as an inactive precursor and is activated as a result of proteolysis in the Golgi apparatus. [7] The extracellular part of the protein consists of 8 consecutive carbohydrate-recognizing domains of type C (CRD), type II fibronectin domain and cysteine-enriched domain. The cytoplasmic domain has no signal sequences and is not capable of signal transmission per se. [eight]
The N-terminal cysteine-enriched domain is homologous to the ricin B chain and binds to sulfated saccharides, and binds with especially high affinity to N-acetylgalactosamine and galactose sulfated at positions 3 and 4 of the pyranose ring. [9] Other ligands of this domain are chondroitin sulfates A and B, sulfated sialyl Lewis X and sialyl Lewis A. [6] The mannose receptor is the only member of the family in which the cysteine-enriched domain is functionally active. [five]
Glycosylation
The mannose receptor is highly glycosylated ; its N-glycosylation sites show a high degree of conservatism in humans and mice, which indicates the important role of glycosylated sites. Sialic acid in the glycans of the receptor plays an important role in the binding of sulfated and mannosylated glycoproteins. Sialylation of the mannose receptor regulates its multimerization, which affects the binding of sulfated glycoproteins. [8] [10]
Functions
Types
In humans, there are two genes encoding two types of mannose receptor:
| Gene | Protein | Synonyms |
|---|---|---|
| MRC1 | Mannose macrophage receptor 1 | Mannose receptor type C, 1, protein of the family with lectin C type domain 13D ( CLEC13D ), CD206, MMR |
| MRC2 | Mannose macrophage receptor 2 | Mannose receptor type C, 2, A protein associated with the urokinase type plasminogen activator receptor, Cd280 |
Notes
- ↑ Szolnoky G., Bata-Csörgö Z., Kenderessy AS, Kiss M., Pivarcsi A., Novák Z., Nagy Newman K., Michel G., Ruzicka T., Maródi L., Dobozy A., Kemény L. A mannose-binding receptor is expressed on human keratinocytes and mediates killing of Candida albicans (English) // Journal of Investigative Dermatology : journal. - 2001 .-- August ( vol. 117 , no. 2 ). - P. 205-213 . - DOI : 10.1046 / j.1523-1747.2001.14071.x . - PMID 11511295 .
- ↑ Sheikh H., Yarwood H., Ashworth A., Isacke CM Endo180, an endocytic recycling glycoprotein related to the macrophage mannose receptor is expressed on fibroblasts, endothelial cells and macrophages and functions as a lectin receptor ( Journal ) // Journal of Cell Science : journal. - The Company of Biologists 2000 .-- March ( vol. 113 (Pt 6) ). - P. 1021-1032 . - PMID 10683150 .
- ↑ East L., Isacke CM The mannose receptor family (Eng.) // Biochimica et Biophysica Acta : journal. - 2002. - Vol. 1572 , no. 2-3 . - P. 364-386 . - DOI : 10.1016 / S0304-4165 (02) 00319-7 . - PMID 12223280 .
- ↑ Schlesinger PH, Doebber TW, Mandell BF, White R., DeSchryver C., Rodman JS, Miller MJ, Stahl P. Plasma clearance of glycoproteins with terminal mannose and N-acetylglucosamine by liver non-parenchymal cells. Studies with beta-glucuronidase, N-acetyl-β-D-glucosaminidase, ribonuclease B and agalacto-orosomucoid (English) // Biochemical Journal : journal. - 1978. - Vol. 176 , no. 1 . - P. 103-109 . - PMID 728098 .
- ↑ 1 2 Lee SJ, Evers S., Roeder D., Parlow AF, Risteli J., Risteli L., Lee YC, Feizi T., Langen H., Nussenzweig MC Mannose receptor-mediated regulation of serum glycoprotein homeostasis ) // Science: journal. - 2002. - Vol. 295 , no. 5561 . - P. 1898-1901 . - DOI : 10.1126 / science.1069540 . - PMID 11884756 .
- ↑ 1 2 Gazi U., Martinez-Pomares L. Influence of the mannose receptor in host immune responses (Eng.) // Immunobiology: journal. - 2009. - Vol. 214 , no. 7 . - P. 554-561 . - DOI : 10.1016 / j.imbio.2008.11.004 . - PMID 19162368 .
- ↑ Stahl PD, Ezekowitz RAB The mannose receptor is a pattern recognition receptor involved in host defense // Current opinion in immunology. - 1998. - T. 10. - No. 1. - S. 50-55.
- ↑ 1 2 Martinez-Pomares L. The mannose receptor (Eng.) // Journal of Leukocyte Biology : journal. - 2012. - Vol. 92 , no. 6 . - P. 1177-1186 . - DOI : 10.1189 / jlb.0512231 . - PMID 22966131 .
- ↑ Fiete DJ, Beranek MC, Baenziger JU A cysteine-rich domain of the "mannose" receptor mediates GalNAc-4-SO4 binding (Eng.) // Proceedings of the National Academy of Sciences of the United States of America : journal. - 1998 .-- March ( vol. 95 , no. 5 ). - P. 2089-2093 . - PMID 9482843 .
- ↑ Su Y., Bakker T., Harris J., Tsang C., Brown GD, Wormald MR, Gordon S., Dwek RA, Rudd PM, Martinez-Pomares L. Glycosylation influences the lectin activities of the macrophage mannose receptor .) // Journal of Biological Chemistry : journal. - 2005. - Vol. 280 , no. 38 . - P. 32811—32820 . - DOI : 10.1074 / jbc.M503457200 . - PMID 15983039 .
Literature
- Azad, AK .; Rajaram, MV .; Schlesinger, LS. Exploitation of the Macrophage Mannose Receptor (CD206) in Infectious Disease Diagnostics and Therapeutics. (English) // J Cytol Mol Biol: journal. - 2014 .-- January ( vol. 1 , no. 1 ). - DOI : 10.13188 / 2325-4653.1000003 . - PMID 24672807 .
- Martinez-Pomares, L. The mannose receptor. (Eng.) // Journal of Leukocyte Biology : journal. - 2012 .-- December ( vol. 92 , no. 6 ). - P. 1177-1186 . - DOI : 10.1189 / jlb.0512231 . - PMID 22966131 .
- Vautier, S .; MacCallum, DM .; Brown, GD. C-type lectin receptors and cytokines in fungal immunity. (English) // Cytokine: journal. - 2012 .-- April ( vol. 58 , no. 1 ). - P. 89-99 . - DOI : 10.1016 / j.cyto.2011.08.0.031 . - PMID 21924922 .