Tris-acetate-EDTA buffer (TAE) is a buffer solution containing Tris , acetic acid and EDTA (ethylenediaminetetraacetic acid).
Used in molecular biology mainly for gel electrophoresis in the separation of nucleic acid fragments. [1] It has less buffer capacity than TBE, due to which it depletes faster, however, linear double-stranded DNA fragments move to TAE faster.
| Component | Amount to prepare 100 ml solution |
|---|---|
| Tris (base) | 24.22 g |
| EDTA (disodium salt) | 1,862 g |
| Acetic acid (ice) | 8.96 ml |
| H 2 O ( deionized ) | 73.3 g |
To obtain a working concentration of 50-fold buffer diluted in distilled water in the ratio of 1:49.
Application
TAE is widely used in gel electrophoresis (as a pore buffer and a component of an agarose gel) for DNA purification, analysis of DNA products obtained by PCR, separation of DNA fragments. [3]
See also
- DNA electrophoresis
- PCR
- EDTA
- TE buffer
Notes
- β Ogden, RC, and Adams, DA, (1987) Electrophoresis in agarose and acrylamide gels. Methods Enzymol ., 152 :, 61-87.
- β Calculate TAE buffer on molbiol.ru . Archived December 13, 2012.
- β Sambrook, Fritsch, and Maniatis (1989) Molecular Cloning: A Laboratory Manual , 2nd ed., Cold Spring Harbor Press, Cold Spring Harbor, New York, volume 3, apendices B.11 and B.23 ISBN 0-87969- 309-6