NLRC4 ( English NLR family, CARD domain containing 4 ) is a cytosolic protein , a Nod-like receptor from the NOD subfamily, a product of the NLRC4 gene. Gene cloned in 2001. [1] Plays a role in the activation of apoptosis .
| NLRC4 | |
|---|---|
| Designations | |
| Characters | NLRC4 ; CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, ipaf, CARD12 |
| Entrez gene | 58484 |
| Hgnc | 16412 |
| OMIM | 606831 |
| Refseq | NM_001199138 |
| UniProt | Q9NPP4 |
| Other data | |
| Locus | 2nd hr. , 2p22 p21 |
Content
Functions
Plays a role in the activation of caspase-1 in response to bacterial pathogens ( lipopolysaccharide , flagellin , PrgJ).
Structure
The mature protein consists of 1024 amino acids , the molecular weight is 116.2 kDa . The molecule includes a CARD domain, a NACHT domain with an ATP binding site, and 14 LRR ( leucine- enriched) repeats.
As a result of alternative splicing , 4 isoforms are formed.
Interacts through CARD-domains with ASC, NOD2 , BCL10, NALP1 , caspase-1 and CARD15 . [2]
Notes
- ↑ Damiano JS, Stehlik C., Pio F., Godzik A., Reed JC CLAN, a novel human CED-4-like gene (Unidentified) // Genomics . - Academic Press , 2001. - July ( t. 75 , № 1-3 ). - p . 77-83 . - DOI : 10.1006 / geno.2001.6579 . - PMID 11472070 .
- ↑ UniProt Q9NPP4
Literature
- Kofoed EM, VAN RE RE REQUESTS INELIMESome specificity (Engl.) // Nature: journal. - 2011. - September ( vol. 477 , no. 7366 ). - P. 592-595 . - DOI : 10.1038 / nature10394 . - PMID 21874021 .
- Poyet JL, Srinivasula SM, Tnani M., et al. Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1. (English) // Journal of Biological Chemistry : journal. - 2001. - Vol. 276 , no. 30 - P. 28309-28313 . - DOI : 10.1074 / jbc.C100250200 . - PMID 11390368 .
- Damiano JS, Stehlik C., Pio F., et al. CLAN, a novel human CED-4-like gene. (Neopr.) // Genomics . - Academic Press , 2001. - Vol. 75 , No. 1-3 . - p . 77-83 . - DOI : 10.1006 / geno.2001.6579 . - PMID 11472070 .
- Strausberg RL, Feingold EA, Grouse LH, et al. Human and mouse cDNA sequences. (Eng.) // Proceedings of the United States of America : journal. - 2003. - Vol. 99 , no. 26 - P. 16899-16903 . - DOI : 10.1073 / pnas.242603899 . - PMID 12477932 .
- Fu WN, Bertoni F., Kelsey SM, et al. Role of DNA methylation in the suppression of Apaf-1 protein in human leukaemia. (English) // Oncogene : journal. - 2003. - Vol. 22 , no. 3 P. 451-455 . - DOI : 10.1038 / sj.onc.1206147 . - PMID 12545166 .
- Clark HF, Gurney AL, Abaya E., et al. The secreted protein discovery initiative (SPDI), a large-scale effort to identify and transmembrane proteins: a bioinformatics assessment. (English) // Genome Research : journal. - 2003. - Vol. 13 , no. 10 - P. 2265-2270 . - DOI : 10.1101 / gr.1293003 . - PMID 12975309 .
- Ota T., Suzuki Y., Nishikawa T., et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. (Eng.) // Nature Genetics : journal. - 2004. - Vol. 36 , no. 1 . - P. 40—5 . - DOI : 10.1038 / ng1285 . - PMID 14702039 .
- Agostini L., Martinon F., Burns K., et al. NALP3 forms an IL-1beta-processing inflammasome with auto-inflammatory disorder. (English) // Immunity: journal. - Cell Press , 2004. - Vol. 20 , no. 3 - p . 319-325 . - DOI : 10.1016 / S1074-7613 (04) 00046-9 . - PMID 15030775 .
- Damiano JS, Oliveira V., Welsh K., Reed JC Heterotypic interactions among NACHT domains: regulation of innate immune responses. (Eng.) // Biochemical Journal : journal. - 2004. - Vol. 381 , no. Pt 1 . - P. 213-219 . - DOI : 10.1042 / BJ20031506 . - PMID 15107016 .
- Gerhard DS, Wagner L., Feingold EA, et al. The cIvA project: the Mammalian Gene Collection (MGC). (English) // Genome Research : journal. - 2004. - Vol. 14 , no. 10b . - P. 2121-2127 . - DOI : 10.1101 / gr.2596504 . - PMID 15489334 .
- Damiano JS, Newman RM, Reed JC Multiple roles of CLAN (caspase-associated recruitment domain, leucine-rich repeat, and NAIP CIIA HET-E, and TP1-containing protein) in the mammalian innate immune response. (English) // Journal of Immunology : journal. - 2004. - Vol. 173 , no. 10 - P. 6338–6345 . - PMID 15528373 .
- Sadasivam S., Gupta S., Radha V., et al. Caspase-1 activator Ipaf is a p53-inducible gene involved in apoptosis. (English) // Oncogene : journal. - 2005. - Vol. 24 , no. 4 - P. 627-636 . - DOI : 10.1038 / sj.onc.1208201 . - PMID 15580302 .
- Lu C., Wang A., Wang L., et al. Nucleotide binding to CARD12 caspase-1 activation. (English) // Biochemical and Biophysical Research Communications : journal. - 2005. - Vol. 331 , no. 4 - P. 1114-1119 . - DOI : 10.1016 / j.bbrc.2005.04.027 . - PMID 15882992 .
- Rual JF, Venkatesan K., Hao T., et al. Towards a proteome-scale map of the human protein-protein interaction network. (English) // Nature: journal. - 2005. - Vol. 437 , no. 7062 . - P. 1173-1178 . - DOI : 10.1038 / nature04209 . - PMID 16189514 .
- Thalappilly S., Sadasivam S., Radha V., Swarup G. Involvement of caspase 1 and its activator Ipaf upstream of mitochondrial events in apoptosis. (English) // FEBS Journal : journal. - 2006. - Vol. 273 , no. 12 - P. 2766-2778 . - DOI : 10.1111 / j.1742-4658.2006.05293.x . - PMID 16817903 .