General scheme of the method
Northern blot ( English Northern blot ) - a method for studying gene expression by testing RNA molecules (mRNA) and their fragments in samples.
The Northern blot method was proposed in 1977 by Stanford University employees James Alvine, David Kemp, and George Stark [1] and named by its analogy with the Southern blot - the first method of this type proposed by Edwin Southern [2] . The main difference between the Northern blot method and the Southern blot method is that the detected substrate is not DNA, but RNA. This leads to differences in the method: instead of nitrocellulose, a filter made of diazobenzyloxymethyl cellulose is used, complementary DNA molecules are used as probes, etc.
Notes
- ↑ Alwine JC, Kemp DJ, Stark GR Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes (English) // Proceedings of the National Academy of Sciences of the United States of America : journal . - 1977. - Vol. 74 , no. 12 . - P. 5350-5354 . - DOI : 10.1073 / pnas.74.12.5350 . - PMID 414220 .
- ↑ Alberts, B., Johnson, A., Lewis, J. Raff, M., Roberts, K., Walter, P. 2008. Molecular Biology of the Cell, 5th ed. Garland Science, Taylor & Francis Group, NY, pp 538-539.