Lactate dehydrogenase (L-lactate: NAD oxidoreductase (LDH) 1.1.1.27) is an enzyme involved in glycolysis reactions. Lactate dehydrogenase catalyzes the conversion of lactate to pyruvate , with the formation of NADH.
| Lactate dehydrogenase | |
|---|---|
| Identifiers | |
| Cipher cf | 1.1.1.27 |
| CAS Number | 9001-60-9 |
| Enzyme bases | |
| Intenz | Intenz view |
| BRENDA | BRENDA entry |
| Expasy | NiceZyme view |
| Metacyc | metabolic pathway |
| Kegg | KEGG entry |
| PRIAM | profile |
| PDB structures | RCSB PDB PDBe PDBj PDBsum |
| Gene ontology | AmiGO • EGO |
| Search | |
| PMC | articles |
| PubMed | articles |
| NCBI | NCBI proteins |
| Cas | 9001-60-9 |
| Lactate Dehydrogenase A (subunit M) | |
|---|---|
 Lactate dehydrogenase M 4 (an isoenzyme found in skeletal muscle ). PDB 1I10 . | |
| Designations | |
| Characters | LDHA ; LDHM |
| Cas | |
| Entrez gene | 3939 |
| Hgnc | 6535 |
| Omim | 150,000 |
| Refseq | NM_005566 |
| Uniprot | P00338 |
| Other data | |
| Cipher cf | 1.1.1.27 |
| Locus | 11th , 11p15.4 |
| Lactate Dehydrogenase B (subunit H) | |
|---|---|
| Designations | |
| Characters | LDHB ; LDHL |
| Cas | |
| Entrez gene | 3945 |
| Hgnc | 6541 |
| Omim | 150100 |
| Refseq | NM_002300 |
| Uniprot | P07195 |
| Other data | |
| Cipher cf | 1.1.1.27 |
| Locus | 12th , 12p12.2-12.1 |
| Lactate Dehydrogenase C subunit | |
|---|---|
| Designations | |
| Characters | LDHC |
| Cas | |
| Entrez gene | 3948 |
| Hgnc | 6544 |
| Omim | 150150 |
| Refseq | NM_002301 |
| Uniprot | P07864 |
| Other data | |
| Cipher cf | 1.1.1.27 |
| Locus | 11th , 11p15.5-15.3 |
Lactate dehydrogenase was first obtained from the washed muscle by Maerhof in 1909 , and in 1940, Straub received it in crystalline form.
Lactate dehydrogenase is resistant to chemical influences, such as reactants that oxidize or block sulfhydryl groups , such as iodoacetate and iodobenzoate . The activity of lactate dehydrogenase decreases with an increase in the concentration of pyruvate above 10-4 M. Significant inactivation, reversible with the addition of cysteine , occurs when incubated with parachloro mercuribenzoate .
The coenzyme of lactate dehydrogenase is nicotinamide adenine dinucleotide , abbreviated NAD. NAD is a dinucleotide-type coenzyme in which two nucleoside-5'-monophosphates are connected by a phosphoanhydride bond.
The LDH test is included in the biochemical blood test . Norms:
- newborns - up to 2000 units / l
- children under 2 years old - 430 units / l
- children from 2 to 12 - 295 units / l
- children over 12 years old and adults - 250 units / l