Bromodeoxyuridine ( Bromodeoxyuridine ), abbreviated as BDU ( BrdU ) is a synthetic nucleoside , an analog of thymidine , used to detect proliferating cells in living tissues [1] , to study DNA replication [2] , for differential staining of sister chromatids [3] .
| Bromodeoxyuridine | |
|---|---|
 | |
| Are common | |
| Abbreviations | BDU |
| Traditional names | 2'-deoxy-5-bromuridine |
| Chem. formula | C 9 H 11 BrN 2 O 5 |
| Physical properties | |
| Molar mass | 307.098 g / mol |
| Classification | |
| Reg. CAS number | 59-14-3 |
| PubChem | |
| Reg. EINECS number | |
| Smiles | |
| Inchi | |
| Chebi | |
| ChemSpider | |
Hippocampal neurogenesis : NOS allows for the identification of proliferating cells in the subgranular zone of the dentate gyrus
Bromodeoxyuridine is able to replace thymidine in the process of DNA replication, integrating into new DNA. Immunohistochemical staining with antibodies to bromodeoxyuridine allows detecting the incorporated modified nucleoside, thereby revealing proliferating cells. For successful immunohistochemical detection of incorporated bromodeoxyuridine, DNA must be denatured.
Bromodeoxyuridine is a fairly potent mutagen [4] , and therefore requires caution in handling.
Notes
- ↑ Wilson GD et al. Measurement of cell kinetics in human tumors in vivo using bromodeoxyuridine incorporation and flow cytometry // British journal of cancer. - 1988. - T. 58. - No. 4. - S. 423.
- ↑ Gratzner HG Monoclonal antibody to 5-bromo-and 5-iododeoxyuridine: a new reagent for detection of DNA replication // Science. - 1982. - T. 218. - No. 4571. - S. 474-475.
- ↑ Ikushima T., Wolff S. Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine-and 5-iododeoxyuridine substituted Chinese hamster chromosomes // Experimental cell research. - 1974. - T. 87. - No. 1. - S. 15-19.
- ↑ Aebersold PM Mutagenic mechanism of 5-bromodeoxyuridine in Chinese hamster cells // Mutation Research / Fundamental and Molecular Mechanisms of Mutagenesis. - 1976. - T. 36. - No. 3. - S. 357-362.
See also
- Doublecortin is also used as a marker of neurogenesis .
- 5-bromouracil - experimental mutagen
Links
- DNA replication embedding - multimedia lecture by Jiayang Chien, Wellesley College