Cyclonic is a salt solution of monoclonal antibodies to antigens located on the surface of human erythrocytes. Monoclonal antibodies for colicones are produced using hybrids, or certain bacterial strains . Bacteria for such industries are obtained by genetic engineering .
The name of the drugs comes from the name of the institute where they invented and manufactured these solutions - the Central Order of Lenin Institute of Clinical Oncology in Moscow, now the Hematological Research Center of the Federal Agency for Health and Social Development .
Content
Characteristics and basic properties of anti-A, anti-B and anti-AB
Monoclonal Anti-A and Anti-B antibodies are produced by two mouse hybridomas and belong to the class M immunoglobulins. The cyclones are made from ascites fluid of anti-A and anti-B hybrid mice, the anti-AB polyclone is a mixture of monoclonal anti-A and anti - In antibodies. Manufacturing technology reagent eliminates the possibility of contamination of human pathogenic viruses.
Technique for determining the human blood groups of an ABO system using cyclones
The determination is made in native blood taken as a preservative; in blood taken without preservative; in blood taken from a finger. The method of direct hemagglutination on the plane is used: on a plate or tablet. Blood type is determined in a room with good lighting at a temperature of 15-25 Β° C. 1. Anti-A, anti-B, and anti-AB cyclones are applied to the plate or plate with individual pipettes in one large drop (0.1 ml) under appropriate inscriptions. Next to the drops of antibodies, one small drop of the test blood is applied (0.01-0.03 ml). 3. Blood is mixed with reagent. Next, the reaction with the cyclones is monitored with a slight rocking of the plate or tablet for 3 minutes. Agglutination of erythrocytes with cyclones usually occurs in the first 3-5 seconds, but observation should be carried out for 3 minutes. - due to the later appearance of agglutination with red blood cells containing weak varieties of antigens A or B. The result of the reaction in each drop may be positive or negative. A positive result is expressed in agglutination (gluing) of erythrocytes. Agglutinates are visible to the naked eye in the form of small red aggregates that quickly merge into large flakes. With a negative reaction, the drop remains evenly colored red, agglutinates are not detected in it.
In practice, polyclones are used to determine blood groups . Currently, there are a large number of cyclones to different antigens, which are designed to determine blood groups for various systems. The most commonly used are cyclones anti-A (red liquid), anti-B (blue liquid) and anti-D (transparent liquid). The first two tsoliklon intended to determine the blood group on the system AB0, the last - to determine the blood group on the system Rh. In addition, it is quite common to use anti-A1 (lectin) tsikrylon, which allows among carriers of the A-antigen to select a population of carriers of the weakened A2-antigen (that is, individuals with A2 (II) or A2B (IV) blood type). Non-use of the anti-A1 polyclone is fraught with an incorrect result for this category of persons (it is likely that 0 (I) is put instead of A2 (II) and B (III) instead of A2B (IV) blood groups). Coliclon anti-D has 2 modifications: anti-D itself, used in medical diagnostic practice in combination with 10% gelatin , and anti-D-super, allowing for the rapid determination of the Rh factor (all Rh-negative samples require compulsory check with anti-D ziklonon, as it is possible to have a carrier of a weak Rh system antigen).
Release form
The cyclones are produced in liquid form in plastic bottles of 10 ml with a dropper or in glass bottles of 5 ml. Anti-A coliclone is red, Anti-B blue and Anti-AB are colorless. Sodium azide at a final concentration of 0.1% is used as a preservative.
Storage
Shelf life - 2 years at a temperature of 2-8 Β° C. Opened vial can be stored at 2-8 Β° C for a month in closed form.
Literature
- Instructions for use of COLICLONS Anti-A, Anti-B and Anti-AV diagnostic liquid for the determination of human blood groups of the ABO system (Approved by Order No. 2 of the Ministry of Health of the Russian Federation of 05.10.2000)